The lack of comprehensive knowledge of canine blood types and the inability to type beyond DEA 1 means that cross matching is necessary to detect serious antibody led incompatibilities.

Cross matching is an in vitro test mixing donor and recipient components (blood or plasma) to look for potential reactions. Cross matching test result assessment involves looking for agglutination or haemolysis reactions.

This test looks for those reactions that could cause severe complications. It is best practice to both type and cross match for any transfusion. However, for second and subsequent transfusion in dogs, it is mandatory at any time >4-7 days post the initial transfusion. This is the time it takes for a recipient to produce alloantibody, i.e antibody against foreign red cell antigens.

The lack of comprehensive knowledge of canine blood types and the inability to type beyond DEA 1 means that cross matching is necessary to detect serious antibody led incompatibilities.